The Wickman lab uses multi-disciplinary approaches incorporating state-of-the-art approaches in molecular and cellular biology, protein biochemistry, electrophysiology, chemogenetics, and behavioral analysis to understand how cell excitability contributes to the normal function of the heart and brain. Techniques and approaches utilized in ongoing lab projects include:

Molecular genetics, cell biology, and biochemistry

Molecular biology (cloning/sub-cloning, PCR, RT-PCR and qRT-PCR)
Immunoblotting, immunoprecipitation, and immunohistochemistry
Recombinant protein generation and purification
Membrane trafficking (surface biotinylation)
Cell culture (primary cultures of neurons and cardiac myocytes)
Virus production (AAV and lentivirus)
Conditional gene ablation and RNAi-dependent knockdown


Whole-cell voltage- and current-clamp (brain slices and cultured cells)
Spontaneous activity (action potentials, miniature synaptic currents)
Evoked/synaptic postsynaptic currents
Single channel analysis

Organ physiology and behavior

Cardiac physiology (ECG telemetry, isolated heart preparation)
Reward-related behavior (self-administration, conditioned place preference, motor activity)
Learning/Memory (fear conditioning, Barnes maze)
Mood-related behavior (elevated plus maze, tail-suspension and forced swim tests)
Nociception and analgesia (warm-water tail-flick, hot-plate)

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